Morphogenesis of Bacteriophage T<sub>4</sub> Head
نویسندگان
چکیده
منابع مشابه
Bacteriophage T4 head morphogenesis. Isolation, partial characterization, and fate of gene 21-defective tau-particles.
A lysozyme-detergent procedure was developed for isolation of tau-particles from cells infected by gene-21 mutants of T4 bacteriophage. These particles have a sedimentation coefficient of 440 +/- 10 S. They contain less than 1% detectable nuclease-resistant DNA, are smaller (650 x 850 A) than normal bacteriophage heads (800 x 1100 A), and exhibit two major bands on 7.5% Na dodecyl sulfate-acryl...
متن کاملMorphogenesis of bacteriophage T4 in extracts of mutant-infected cells.
The complex structure of bacteriophage T4 includes a variety of proteins' which become assembled into mature particles during intracellular development of the virus. Some insight into the genetic control of this process has been provided by physiological studies with conditional lethal mutants, which show that over 40 phage genes are involved in T4 morphogenesis2 (Fig. 1). However, the mechanis...
متن کاملMolecular architecture of the prolate head of bacteriophage T4.
The head of bacteriophage T4 is a prolate icosahedron with one unique portal vertex to which the phage tail is attached. The three-dimensional structure of mature bacteriophage T4 head has been determined to 22-A resolution by using cryo-electron microscopy. The T4 capsid has a hexagonal surface lattice characterized by the triangulation numbers T(end) = 13 laevo for the icosahedral caps and T(...
متن کاملStructure determination of the head-tail connector of bacteriophage phi29.
The head-tail connector of bacteriophage phi29 is composed of 12 36 kDa subunits with 12-fold symmetry. It is the central component of a rotary motor that packages the genomic dsDNA into preformed proheads. This motor consists of the head-tail connector, surrounded by a phi29-encoded, 174-base, RNA and a viral ATPase protein, both of which have fivefold symmetry in three-dimensional cryo-electr...
متن کاملBacteriophage of Pseudomonas aeruginosa with unfamiliar head morphology.
P. aeruginosa (Kitasato strain) was used as host. Forty-three strains of P. aeruginosa from the culture collection of the Department of Bacteriology, School of Medicine, Kyushu University were used for determination of the lytic spectrum of the phage. A phage of P. aeruginosa with rod-shaped head was designated as K. The host cells were cultivated in nutrient broth with aeration for 3 hr. At th...
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ژورنال
عنوان ژورنال: Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
سال: 1975
ISSN: 0030-1558,1882-4528
DOI: 10.4044/joma1947.87.5-6_529